EFFECT OF CYTOKININ-ACTIVE PHENYLUREA DERIVATIVES ON SHOOT MULTIPLICATION, PEROXIDASE AND SUPEROXIDE DISMUTASE ACTIVITIES OF in vitro CULTURED CARNATION

In the present study examined the effects of different types of cytokinins on the growth of in vitro cultured carnation. NAA alone produced neither multiplication nor elongation. Combined with BAP, NAA increased multiplication. The biological activity of 4-PU-30 and especially that of thidiazuron was considerably higher than BAP’s activity. In equal concentrations with BAP thidiazuron increased more than 6 times the number of shoots and about 5 times the number of explants per culture in cv. White Sim. All tested concentrations of 4-PU-30 and thidiazuron increased the fresh weight per culture in cv. Red Lena, more than BAP. Application of 4-PU-30 and thidiazuron induced changes in all fractions of peroxidase but soluble peroxidase had highest effect. New bands only be seen after application of both 4μM and 0.4μM 4-PU-30. SOD activity was stimulated by BAP. All studied concentrations of thidiazuron and 4-PU-30 also increased SOD activity. Strongest increase in SOD activity is in the highest concentration of thidiazuron.